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In one technique, the pIII (“P-three”) protein of one of these organisms allows it to attach to the F pilus of a pathogen. For 10 points each:
[10m] Name these organisms used in a namesake “assisted continuous evolution” technique. Libraries from these organisms undergo multiple rounds of affinity selection followed by amplification in a namesake “display.”
ANSWER: bacteriophages [accept phage display or phage-assisted continuous evolution; accept lambda phage or M13 phage; prompt on viruses]
[10e] The M13 phage used in phage-assisted continuous evolution replicates by infecting this pathogen. Hershey and Chase used a T2 phage to infect this rod-shaped Gram-negative bacteria that can cause food poisoning.
ANSWER: E. coli [or Escherichia coli]
[10h] The M13 phage is also used in Kunkel’s method of performing this technique using mutated E. coli strains. Either this technique or error-prone PCR is used to generate libraries in alanine scanning.
ANSWER: site-directed mutagenesis [or site-specific mutagenesis or oligonucleotide-directed mutagenesis; prompt on SDM or mutagenesis or point mutation or mutation scanning or deep mutation scanning]
<Waterloo B, Biology>

EditionsHeardPPBEasy %Medium %Hard %
15416.1194%54%13%

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Conversion

TeamOpponentPart 1Part 2Part 3TotalParts
Bruin AGeorgia Tech A1010020ME
Georgia Tech BAlabama A010010E
Georgia Tech CTennessee A1010020ME
Vanderbilt AGeorgia Tech D010010E

Summary

TournamentEditionHeardPPBEasy %Medium %Hard %
California2025-02-01320.00100%100%0%
Florida2025-02-01310.0067%33%0%
Great Lakes2025-02-01613.33100%33%0%
Midwest2025-02-01613.3367%50%17%
North2025-02-01215.00100%50%0%
Northeast2025-02-01316.67100%67%0%
Overflow2025-02-01520.00100%60%40%
South Central2025-02-01220.00100%50%50%
Southeast2025-02-01415.00100%50%0%
UK2025-02-011015.00100%40%10%
Upper Mid-Atlantic2025-02-01722.86100%100%29%
Upstate NY2025-02-01310.00100%0%0%