Bonus

In one technique, the pIII (“P-three”) protein of one of these organisms allows it to attach to the F pilus of a pathogen. For 10 points each:
[10m] Name these organisms used in a namesake “assisted continuous evolution” technique. Libraries from these organisms undergo multiple rounds of affinity selection followed by amplification in a namesake “display.”
ANSWER: bacteriophages [accept phage display or phage-assisted continuous evolution; accept lambda phage or M13 phage; prompt on viruses]
[10e] The M13 phage used in phage-assisted continuous evolution replicates by infecting this pathogen. Hershey and Chase used a T2 phage to infect this rod-shaped Gram-negative bacteria that can cause food poisoning.
ANSWER: E. coli [or Escherichia coli]
[10h] The M13 phage is also used in Kunkel’s method of performing this technique using mutated E. coli strains. Either this technique or error-prone PCR is used to generate libraries in alanine scanning.
ANSWER: site-directed mutagenesis [or site-specific mutagenesis or oligonucleotide-directed mutagenesis; prompt on SDM or mutagenesis or point mutation or mutation scanning or deep mutation scanning]
<Waterloo B, Biology>

EditionsHeardPPBEasy %Medium %Hard %
15416.1194%54%13%

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Conversion

TeamOpponentPart 1Part 2Part 3TotalParts
ASUTexas A10101030MEH
BUHarvard B010010E
BristolImperial B010010E
Bruin AGeorgia Tech A1010020ME
Cambridge AImperial A1010020ME
Cambridge BOxford C1010020ME
Cambridge COxford B1010020ME
Cambridge DOxford A010010E
Cambridge ESouthampton B10101030MEH
Carnegie Mellon AMichigan State1010020ME
Case Western AOhio State B010010E
Case Western BCarnegie Mellon B010010E
Chicago AMissouri1001020MH
Chicago BMissouri S&T0000
Chicago DIndiana B010010E
Claremont AUCSD1010020ME
Columbia BGeorge Washington B10101030MEH
Cornell BVassar A1010020ME
Cornell DESF A010010E
Florida AFlorida State A1010020ME
George Washington AYale A1010020ME
Georgia Tech BAlabama A010010E
Georgia Tech CTennessee A1010020ME
Harvard ATufts A1010020ME
Illinois AWashU A1010020ME
Indiana AChicago C1010020ME
Iowa StateWinona State1010020ME
Johns Hopkins AColumbia C10101030MEH
LSESouthampton A010010E
MIT ABrown A1010020ME
ManchesterDurham010010E
Maryland AHaverford B1010020ME
Michigan AMichigan D1010020ME
Michigan BKenyon010010E
Michigan COhio State A010010E
Minnesota BWisconsin B010010E
NYU AMaryland B1010020ME
NYU CWarwick A010010E
Northwestern AGeorgetown A10101030MEH
Notre Dame AIowa1010020ME
Notre Dame BGeorgetown B010010E
Notre Dame CPurdue010010E
Penn AJohn Jay College1010020ME
RIT ACornell C010010E
RIT BBinghamton A010010E
SheffieldWarwick B010010E
Stanford BNorthwestern B10101030MEH
TAMUTexas B010010E
UC Berkeley AUCLA1010020ME
UC Berkeley BClaremont B1010020ME
UCF CUCF B0000
Valencia AFlorida B010010E
Vanderbilt AGeorgia Tech D010010E
WashU BIllinois B010010E

Summary

TournamentEditionHeardPPBEasy %Medium %Hard %
California2025-02-01320.00100%100%0%
Florida2025-02-01310.0067%33%0%
Great Lakes2025-02-01613.33100%33%0%
Midwest2025-02-01613.3367%50%17%
North2025-02-01215.00100%50%0%
Northeast2025-02-01316.67100%67%0%
Overflow2025-02-01520.00100%60%40%
South Central2025-02-01220.00100%50%50%
Southeast2025-02-01415.00100%50%0%
UK2025-02-011015.00100%40%10%
Upper Mid-Atlantic2025-02-01722.86100%100%29%
Upstate NY2025-02-01310.00100%0%0%