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Preventing the use of rotationally symmetric static fields in this technique can help overcome a limit set by Scherzer’s theorem. The “spot-scan” technique is sometimes used to prevent beam-induced motion in this larger technique. Samples in this technique may use heavy metals (-5[1])such as tungsten and osmium to undergo a “negative stain.” The development of a form of this technique, for which Dubochet, Frank, and Henderson were awarded the 2017 Nobel Prize in Chemistry, generated a “resolution revolution.” In a form of this technique, the observed specimen is embedded in vitrified ice before being mounted on a grid and fixed via plunging in liquid ethane. For 10 points, (10[1])name this imaging technique that comes in “scanning,” (10[1])“transmission,” and “cryogenic” (10[1])types. ■END■ (10[2]0[2])

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